Anatomy and histology of hematopoietic tissues
[ Fish ]
1. Osteichthyes (3/28)
--- Lecture note
--- Thymus
Head kidney
Spleen
--- Smeared blood films
Anatomy and histology
of hematopoietic tissues
[ Fish ]
1. Osteichthyes (3/28)
---
Lecture note
--- Thymus
Head
kidney
Spleen
---
Smeared blood films
2. Chondrichthyes (3/28)
1) Spleen
---
Less developed white pulp
---
Small lymphocyte
RBC
Ellipsoids
---
Erythropoiesis and antibody production
2) Thymus
---
Multi-lobulated/ Able to differentiate into cortex and medulla
---
Small lymphocyte as a main
Medium-sized
lymphocyte
Reticular
cell
Large
eosinophilic cell
Myoid
cell
Hassal's
body
3) Leydig and epigonal organs
---
Nearly the same components between two
---
Eosinophilic leukocyte
Plasma
cell
Promyelocyte
Lymphoblast
--- Generally no function
in erythropoiesis
No
Hassal's bodies generally proved
3. Cyclostome [ should be tried to get histological and ultrastructural
photoes ]
1) Primitive or
intestinal spleen in myxiniformes
(1) Granular cell as a main
---
Corresponding to neutrophil
(2) Reticular
cell
(3) Undifferentiated hemocyte or blood
cells
2) Circulating blood
---
RBCs
in myxiniformes suggesting that they are possibley produced within the circulating blood
3)
Pronephric central loci, 전신중심소
---
Cellular components
--- RBC
Lymphocyte
Granular cell
Phagocyte
---
No plasma cell within it
3) Hemopoiesis in lamprey
---
Hemopoietic function remains only in ammocoetes and immature stages
---
Eventual loss of hemopoietic function
---
Non-parasitic period
---
Loss after metamorphosis
---
Parasitic period
--- Loss
since the time of liver-ascending
---
Intestinal typhlosole
---
Longitudianl elevation of mucosa
---
Having sinusoids
---
Granulocyte
as a main
RBC
Macrophage
Plasma
cell
Lymphocyte
corresponding to mammalian B cell
[ Invertebrates ]
1) Molluscans
-
Bivalvia
- Clam/
Mussel/ Scallops/ Oysters
-
Hemopoietic tissue and hemocytes
---
Arising form differentiation of connective tissue cells
---
But no stem cells nor any cell line of differentiations have been described
or well dfined.
---
Nothing known about the origin of blood pigments
---
Hemoglobin and hemocyanin
---
Divided into hyalinocytes, granular lymphocyte-like cells and granulocytes or
granular cells
---
Subgrouped for granulocytes according to their size of granules
---
Described as ameboid cells
---
A well developed cytoskeleton of actin filaments
#
Pore cells
---
Described as connective tissue cells in Crassostrea gigas (brown cells)and Mytilus edulis
---
Differentiated from the epithleial podocytes
---
Found with hemocytes in Pactinopecten caurinus in large numbers
- Cephalopods
[photos
]
-
Octopus/ Sqpuids/ Fish cuttle
---
Blood constitutes 6% of the body volume in octopods
---
Hemocytes
---
1- 2% of the blood volume
---
Colloids;
---
Hemocyanin/ Glycoproteins of lectin groups/ Lipids/ Enzymes/ Hormones
---
Others
-
A leukopoietic organ of cephalopods known as " the white body "
---
Also refered as the gland of Henson, gland of Faussek and the corps blanc
---
Orbital pits behind the eyes
---
Consisting of several lobes
---
Histological and ultrastructural informations are available
---
Interconnected between lobes by a sheet of collagen
---
Lobes
---
Extensively folded, forming cords
---
Lumen of them
---
Mature leukocytes consisting of 2 major cell types
---
Hemocytoblasts
---
Reticulum cell with large cytoplasmic volume, abundant rER
and nucleoli
---
Leukoblasts
---
Transformed from hemocytoblasts with decreases of cytoplasmic
volume and of
nuclear size
---
Found in groups joined by intercellular bridges
---
Mature leukoblasts (secondary leukoblasts)
---
Large cell size/ Reorganization and folding of the nucleus/
Well-formed Golgi
apparatus/ Remnants
of rER
---
Sinuses of the white body
---
Detachment of leukocytes into them from cords
---
Connecting to the circulatory system
-
Hemocytes
---
Granulocytes or amebocytes as synonymous terms
---
Morphologically seem to be of one type
-
Optic gland stellate cells of octopuses
-
Vacuolized round cells
---
Another type of cell possibly involved in defense mechanisms, as well as in
storage
of excretory metabolites
2) Crustacean
- Decapods
and crabs
- Shrimps/
Lobsters/ Crayfish/ Crabs
[
Anatomy ]
- The dorsal part
of the cardiac stomach
---
Lymphoid organs?
Ancillry
sites in certain species
---
Surrounding antennal artery
--- The
base of the first three pairs of maxillipeds in penaeid shrimp
[
Histology ]
-
Hemopoietic nodules or organs in the epigastric region of the penaeid shrimp
[ microscopic photo ]
---
Two types of stem cells
-
Two patterns of organization
1)
Lobular pattern in crabs, crayfish and lobsters, and panaeid shrimp
---
Lobules
---
Composed of stem cells and maturing hemocytes
<---
Bounded by an intimal layer
---
Situated within a sheet of connective tissue overlying the stomach
---
Connective tissue in turn is covered by a thin intimal layer
---
Lobules in the hemopoietic tissue in the coxal segments of the maxillipeds
of
the penaeid shrimp
---
Thicker than those in the other species
---
Up to 12 lobules in depth
---
Situated in spony connective tissue and lined by a thick layer of collagen fibres
2)
Nodular or tubular pattern in penaeid shrimp
---
Nodules
---
Lymphoid organ?
-
If so, these nodules may not be hemopoietic.
---
Composed of highly branched blood vessels, each with developing hemocytes
forming
a thickened wall
3) Urochordates
-
Sea squirt